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One step inoculation

is the direct inoculation of a bag bioreactor with cells that were frozen and stored over a long period of time in a vial with a high cell density (HCD cell bank) or in a bag with big volume (LV cell bank). For the production of cell banks, perfusion processes have proven their efficacy. Intermediate cultivation steps in a spinner flask or a shake flask (Erlenmeyer flask) are dispensable which leads to savings in both time and cost 22.