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Chemically defined medium

is a culture medium by which all media components are defined. It allows a reproducible production procedure and facilitates product purification. Especially the chemically-defined minimal culture media are in line with the trend, due to their cost-saving potential for modern antibody production processes. The difficulties of leachables in single-use bioreactors were so far only described in combination with chemically-defined minimal culture media for the cultivation of CHO cells and polyethylene contact layers. See bDtBPP, CHOMaster medium, CHO XM 111-10 cells, Irgafos 168 and multilayer.